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chicken cd41 61  (OriGene)


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    OriGene chicken cd41 61
    Chicken Cd41 61, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chicken+cd41+61/CD41-CD61+Complex+Mouse+Monoclonal+Antibody/pm38287279-253-50-55
    Average 92 stars, based on 1 article reviews
    chicken cd41 61 - by Bioz Stars, 2026-10
    92/100 stars

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    Article Title: Single-cell RNA-seq mapping of chicken peripheral blood leukocytes.
    Article Snippet: The blood was diluted 1:1 in sterile phosphate buffered saline without Ca2+ and Mg2+ at pH 7 (PBS) and peripheral blood leukocytes were isolated by Ficoll-Paque PLUS (GE Healthcare Life Sciences) gradient centrifugation as previously described [61]. .. Cells were suspended in PBS supplemented with 2% foetal bovine serum (Gibco® #10,082,147, ThermoFisher Scientific) and 1 mM EDTA and the number of thrombocytes in the samples were reduced by immunomagnetic cell separation using the EasySep PE Positive Selection Kit II (#17,684, StemCell Technologies) and PE-conjugated mouse monoclonal antibody to chicken CD41/61 (clone 11C3, #AM05550PE-N, OriGene) according to the StemCell Technologies depletion protocol no. 28,898 as previously described for chicken leukocytes [61]. .. The depleted cell preparations were suspended in PBS with 0.04% bovine serum albumin (#A7030, Sigma-Aldrich), viability of cell preparations was estimated by trypan blue exclusion to 92–97%.

    Article Title: Single-cell RNA-seq mapping of chicken peripheral blood leukocytes
    Article Snippet: The blood was diluted 1:1 in sterile phosphate buffered saline without Ca 2+ and Mg 2+ at pH 7 (PBS) and peripheral blood leukocytes were isolated by Ficoll-Paque PLUS (GE Healthcare Life Sciences) gradient centrifugation as previously described [ ]. .. Cells were suspended in PBS supplemented with 2% foetal bovine serum (Gibco® #10,082,147, ThermoFisher Scientific) and 1 mM EDTA and the number of thrombocytes in the samples were reduced by immunomagnetic cell separation using the EasySep PE Positive Selection Kit II (#17,684, StemCell Technologies) and PE-conjugated mouse monoclonal antibody to chicken CD41/61 (clone 11C3, #AM05550PE-N, OriGene) according to the StemCell Technologies depletion protocol no. 28,898 as previously described for chicken leukocytes [ ]. .. The depleted cell preparations were suspended in PBS with 0.04% bovine serum albumin (#A7030, Sigma-Aldrich), viability of cell preparations was estimated by trypan blue exclusion to 92–97%.



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    CSF1R -transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R -mApple + cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG -eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R -mApple embryos indicates that transgene expression is restricted to CD45 + (B, green), CSF1R + (C, green) cells in the mesenchyme (red arrowheads) and not CD45 + cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 µm. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R -mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45 + (D, green) and CSF1R + (E, green), but are <t>CD41/61</t> − (F, green). Scale bars in D-F: 100 µm. BV, blood vessel lumen. (G) Scattered eGFP + cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 µm. (H-J) Colocalization of eGFP + cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 µm.
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    CSF1R -transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R -mApple + cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG -eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R -mApple embryos indicates that transgene expression is restricted to CD45 + (B, green), CSF1R + (C, green) cells in the mesenchyme (red arrowheads) and not CD45 + cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 µm. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R -mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45 + (D, green) and CSF1R + (E, green), but are CD41/61 − (F, green). Scale bars in D-F: 100 µm. BV, blood vessel lumen. (G) Scattered eGFP + cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 µm. (H-J) Colocalization of eGFP + cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 µm.

    Journal: Development (Cambridge, England)

    Article Title: Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage

    doi: 10.1242/dev.105593

    Figure Lengend Snippet: CSF1R -transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R -mApple + cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG -eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R -mApple embryos indicates that transgene expression is restricted to CD45 + (B, green), CSF1R + (C, green) cells in the mesenchyme (red arrowheads) and not CD45 + cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 µm. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R -mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45 + (D, green) and CSF1R + (E, green), but are CD41/61 − (F, green). Scale bars in D-F: 100 µm. BV, blood vessel lumen. (G) Scattered eGFP + cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 µm. (H-J) Colocalization of eGFP + cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 µm.

    Article Snippet: Primary antibodies were added: anti-CSF1R ( ); anti-MHC II [clone 2G11 ( )]; anti-chicken CD41/61 (clone 11C3, AbD Serotec); CD45 (clone LT40, SouthernBiotech); anti-Bu-1 (clone L22, AbD Serotec); anti-chicken macrophage subset marker (clone CVI-ChNL-74.2, Prionics); anti-chicken macrophage/monoctyes (clone KUL01, AbD Serotec); and anti-chicken TCR alpha/beta (clone TCR2, AbD Serotec) all diluted by 1/50-1/500 in MST-PBS and sections incubated at 4°C overnight.

    Techniques: Expressing, Staining